Investigation of enantiomer separations in high-performance liquid chromatography with polysaccharide-based chiral selectors and reversed-phase eluents

Author: zoia Shedania
Keywords: Enantiomers, Chirality, Reversed-phase chromatography
Annotation:

Abstract Development of instrumental separation methods of enantiomers was started nearly century ago. Despite the huge amount of experimental data, chiral recognition mechanism is not completely understood yet. Goal of this work is to investigate separations of enantiomers in high-performance liquid chromatography with polysaccharide-based chiral selectors and reversed-phase mobile phases. The factors responsible for changing of elution order have also been studied. For this study 14 chiral sulfoxides were analyzed using 17 chiral stationary phases based on polysaccharide trisphenylcarbamate derivatives, with high chiral recognition ability. The mixture of methanol / water and acetonitrile / water were used as mobile phases. The experiment showed that analysis with methanol / water mobile phase follow reverse-phase chromatography principles, namely, increasing of water content in mobile phase increased the retention time, but not in case of mobile phase of acetonitrile / water mixture. In some cases changes of enantiomer eluation order were also revealed. Column efficiency, resolution and selectivity vary depending on chiral selector and testing substances.