Separation of enantiomers of basic medicines on water-methanol and water- acetonitrile mobile phases in high performance liquid chromatography

Author: Nino Zakashvili
Keywords: b-blockers, polysaccharide
Annotation:

b-Blockers are widely used group of chiral drugs. Separation of enantriomers of chiral drugs is important because the enantiomers commonly exhibit different pharmacological and toxic properties. High-performance liquid chromatography (HPLC) is one of the major methods for separation of enantiomers and polysaccharide derivatives belong to the most successful chiral selectors for separation of enantiomers in HPLC. These materials can be used for HPLC separation of enantiomers in combination with hydrocarbon-alcohol, polar-organic and aqueous-organic mobile phases. The chiral separation mechanism, especially with aqueous-organic mobile phases is not known yet. Therefore, the aim of the present study was to investigate the separation mechanism (hydrophobic or hydrophilic interaction chromatography) of enantiomers of chiral b-blockers on polysaccharide-based chiral columns in combination with aqueous-organic mobile phases. The understanding of chiral separation mechanism will provide the possibility for further optimization of separation process.

Lecture files:

სამაგისტრო ნაშრომი, ნ. ზაქაშვილი [ka]