The effect of flavonoids isolated from different-origin Sapheravi grapes on the antioxidant enzymes of rat liver

Author: Tamuna Mindorashvili
Keywords: flavonoids, antioxidants, superoxid-dismutase, catalase
Annotation:

In recent years, the special interest has been paid to the oxidative stress as one of the negative effects of harmful environmental factors affecting living organisms. The oxidative stress always accompanies the processes like heart attack, stroke, immune reactions to various infections, apoptosis, senile or age-related diseases, etc. To protect from oxidative stress, living organisms have developed powerful anti-oxidative systems which can be enzymatic or nonenzymatic. The antioxidant-defense mechanisms of the organism include the enzymes superoxide dismutase, catalase and glutathione peroxidase as well as nonenzymatic antioxidants, such as vitamins C, K and E and flavonoids. Flavonoids are secondary plant metabolites. The best-described property of every group of flavonoids is their capacity to act as antioxidants. They reduce the intensity of free radical processes. Flavonoids can protect living organisms from oxidative stress by stabilizing the reactive oxygen species. The aim of our research was to study the effect of flavonoid fractions isolated from seeds and skin of Sapheravi grapes on the antioxidant enzymes found in rat liver. The objects under study were flavonoid fractions extracted from the seeds and skin of Sapheravi grapes that had been collected from different regions of Georgia – Khashmi and Jimiti. The two stepped experiment was conducted. At first, liver cells were isolated from the rat and incubated with the initiator agents of lipid peroxidation (e.g. Fe2+) and with flavonoid fractions. Spectrophotometry was used to measure the activity of the enzymes – catalase and superoxide dismutase. The results obtained showed that the flavonoid fractions extracted from the Sapheravi grapes seeds and skin obtained from different regions of Georgia compensate the antioxidant activity of enzymes due to exhibition of their high antioxidative features.

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პრეზენტაცია [ka]
რეფერატი [ka]
აბსტრაქტი [ka]