Study of thermodynamic characteristics of separation of enantiomers in high performance liquid chromatography using polysaccharide based chiral selectors

In this work, it is presented dependency of enantioselectivity on temperature for 4 chiral derivatives of Arylpropionic acid. 6 different chiral stationary phases (CSP) and 2 different mobile phases was used. Main objective of this study was to find which thermodynamic parameter- enthalpy or entropy was mainly influencing separation of enantiomners. Based on collected chromatographic data selectivity- α was calculated, after that Van’t Hoffs graphs (natural logarithm of selectivity lnα versus reverse absolute temperature: 1/T) was plotted. Using Van’t Hoffs plots, difference of enthalpy and entropy change was calculated and based on this it was possible to find out which thermodynamic parameter or parameters influenced enantiomer separation.